Objective: Hemoglobinopathies are an array of disorders that affect hemoglobin (Hb) function. HPLC is by far the most common technique used for detection of hemoglobinopathies worldwide. The aim of this study is to evaluate 3-year retrospective data of a tertiary hospital in Turkey according to prevalence of hemoglobinopathies.

Material and Methods: A three-year data of 2461 patients admitted to our university hospital laboratory were assessed by scanning laboratory and hospital records. Tosoh G8 HPLC instrument was used for screening.

Results: 668 results with HbA2>3.5% and 19 results with 3.5%< HbA2 <4% were detected. 10 of 19 patients were considered as beta-thalassemia. Number of patients with HbA2 ≥4% were 649 and 602 of them were assessed as beta-thalassemia-minor. 25% of patients were assessed as beta-thalassemiaminor, based on HbA2 levels. 391 patient results were found to be HbF>2% and 138 of them with HbA2<3.5% were further investigated. Variant analysis dataset contains 53 patients. Samples with HbA2≤3.5% and HbF≤2% showing variant peaks were scanned and 28 cases were detected. The most common variants were found to be HbH, HbS, HbD, HbC, HbE and HbO-Arab, respectively.

Conclusion: In this study, the most common variants were found to be HbH, HbAS, HbAD, HbAC, HbAE and HbO-Arab, respectively. Moreover, the frequency of beta-thalassemia minor was found as 25% which is higher than general population prevalence of our country. Accompanying diseases especially hematological malignancies, hereditary spherocytosis and IDA should be kept in mind during evaluating hemoglobinopathy analysis.