Journal of Turkish Clinical Biochemistry

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December 01, 2020
JTCB. 2020 Dec 1; 18 (3) : 150-156
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Evaluation of κ and λ light chain expressions by flow cytometry analysis in cases of CLL

Çiğdem Özdemir1, Filiz Yavaşoğlu2, Tülay Köken3
1 Afyonkarahisar Sağlık Bilimleri Üniversitesi Tıp Fakültesi, Tıbbi Patoloji AD., Afyonkarahisar, Türkiye
2 Afyonkarahisar Sağlık Bilimleri Üniversitesi Tıp Fakültesi, İç Hastalıkları AD, Hematoloji BD, Afyonkarahisar, Türkiye
3 Afyonkarahisar Sağlık Bilimleri Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya AD., Afyonkarahisar, Türkiye

Abstract

Objective: Confirmation of the clonality of B lymphocytes by flow cytometry analysis has an important role in the diagnosis of Chronic Lymphocytic Leukemia (CLL). In this retrospective study, we investigated which surface light chain pattern they showed as indicative of the monoclonity of CLL cells.

Material and Methods: Flow cytometry results of 33 CLL patients (12 women / 21 men) were examined retrospectively. The reading results of the samples obtained after the application of the antibodies in the CLL immunophenotyping panel and the lysing procedure were evaluated. Cells that co-express CD19 and CD5 were considered CLL cells, and kappa and lambda light chain expressions were evaluated to the monoclonity of these cells.

Results: When all cases were evaluated, the percentage of cases showing kappa monoclonity was 30.3%, while the percentage of cases showing lambda monoclonity was 51.5%. It was observed that both light chain expressions were absent in 18.2% of cases.

Conclusion: In our patients, lambda light chain restriction was observed most frequently in CLL cells.

Keywords

Chronic Lymphocytic Leukemia; Flow Cytometry; kappa/lambda

Citation

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Özdemir, Çiğdem, Filiz Yavaşoğlu, and Tülay Köken. 2020. “Evaluation of κ and λ Light Chain Expressions by Flow Cytometry Analysis in Cases of CLL”. Journal of Turkish Clinical Biochemistry 18 (3):150-56. https://turkclinbiochem.org/index.php/pub/article/view/376.
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